sumit.bce's picture
Dr. Sumit Kumar Singh
Assistant Professor
School of Bio-chemical Engg., IIT(BHU)
sumit.bce@iitbhu.ac.in
.
Area of Interest: 
Mass spectrometry based glycoproteomics of mAbs and viruses, MD Simulations of protein glycosylation, Multi-Attribute method (MAM) for protein PTMs, protein structure-function relationship.

Education

Ph.D., Indian Institute of Technology Delhi, India
Chemical Engineering

M.Tech., National Institute of Technology, Trichy, India
Chemical Engineering

B.Tech., National Institute of Technology, Raipur, India
Biotechnology

Academic Experience

Assistant Professor (2021- Present)
School of Biochemical Engineering
Indian Institute of Technology (BHU), Varanasi, India

Postdoctoral Researcher (2019-2021)
Department of Chemical and Biomolecular Engineering
University of Delaware, USA
 

 

Publications after joining IIT BHU

Books

1. Sumit K. Singh et al. (Eds.) (2022). Protein Biomarkers: Discovery and Applications in Clinical Diagnostics. Springer, Singapore (Accepted). 

Journal Articles/Book Chapters

  1. Sumit K. Singh, Deepak Kumar, Saurabh Nagpal, Sunil K. Dubey, Anurag S. Rathore (2022).  A charge variant of bevacizumab offers enhanced FcRn-dependent pharmacokinetic half-life and efficacy. Pharmaceutical Research (In press).
  2. Sumit K Singh, Kelvin H Lee (2021) Characterization of monoclonal antibody glycan heterogeneity using hydrophilic interaction liquid chromatography-mass spectrometry. Frontiers in Bioengineering and Biotechnology (In press) DOI : 10.3389/fbioe.2021.805788
  3. Rozaleen Dash, Sumit K Singh, Narendra Chirmule, Anurag S Rathore (2021). Assessment of functional characterization and comparability of biotherapeutics: A review. The AAPS Journal (In press) DOI: 10.1208/s12248-021-00671-0.

 Publications before joining IIT-BHU

  1. Sumit K Singh, Deepak Kumar, Anurag S Rathore (2020). Understanding Oxidation Propensity in GCSF and Assessment of its Safety and Efficacy. Pharmaceutical Research, 37, (207).
  2. Sumit K Singh, A Mishra, N Chirmule, G Goel, Anurag S Rathore (2020) Modulation of granulocyte colony stimulating factor conformation and receptor binding by methionine oxidation. Proteins: Structure, Function and Bioinformatics, 1-13.
  3. Sumit K Singh, D Kumar, Anurag S Rathore (2021) LC-MS based case-by-case analysis of the impact of acidic and basic charge variants of bevacizumab on stability and biological activity. Scientific Reports, 11(1), 1-15.
  4. Avinash Mishra, Rucha Patil, Sumit K Singh, Priyanka Dalal, Anurag S. Rathore (2020) Mechanistic explanation of structural and functional changes induced by methionine mutation in G-CSF protein. Current Research in Biotechnology, 2, 37-44. 
  5. Sumit K Singh, Avinash Mishra, Divyanshi Yadav, Niharika Budholiya, Anurag S Rathore (2019) Understanding the mechanism of copurification of “difficult to remove” host cell proteins in rituximab biosimilar products. Biotechnology Progress, e2936.
  6. Sumit K Singh, G Goel, Anurag S Rathore. (2019). A novel approach for protein identification from complex cell proteome using modified peptide mass fingerprinting algorithm. Electrophoresis,40, (23-24), 3062-3073.
  7. Anurag S Rathore, Dinesh K Yadav, Shyam S Pandey, Sumit K Singh, Deepak Kumar (2018). Preclinical Evaluation of Product Related Impurities and Variants.  Biopharm International, 31 (2), 26-31.
  8. Sumit K Singh, Deepak Kumar, Anurag S Rathore. (2017). Determination of critical quality attributes for a biotherapeutic in the QbD paradigm: GCSF as a case study. The AAPS Journal, 19(6), 1826-1841.
  9. Neh Nupur, Sumit K Singh, Gunjan Narula, Anurag S Rathore (2017). Assessing analytical comparability of biosimilars: GCSF as a case study. Journal of Chromatography B, 1032, 165-171.
  10. Sumit K Singh, Vishwanath Hebbi, Anurag S Rathore, Ira S Krull (2016). Practical Considerations of Capillary Electrophoresis–Mass Spectrometry for Analysis of Biotherapeutics. LC-GC North America, 34(9), 720-728.
  11. Gunjan Narula, Vishwanath Hebbi, Sumit K Singh, Anurag S Rathore. (2016). Glycosylation in mAb therapeutic products: analytical characterization and impact of process. LC-GC Europe, 29(7), 385-392.
  12. Sumit K Singh, G Narula, AS Rathore (2016). Should charge variants of monoclonal antibody therapeutics be considered critical quality attributes? Electrophoresis, 37(17-18), 2338-2346.
  13. Sumit K Singh, Anurag S Rathore (2015). Use of multivariate data analysis in bioprocessing.  Biopharm International, 28(6), 26-31.
  14. Anurag S. Rathore, Sumit K Singh, Mili Pathak, Erik K. Read, Kurt A. Brorson, Cyrus D. Agarabi, Mansoor Khan (2015) Fermentanomics: Relating quality attributes of a monoclonal antibody to cell culture process variables and raw materials using multivariate data analysis, Biotechnology Progress, 31 (6), 1586-1599.
  15. Debamitra Chakravorty, Sumit K. Singh, Parameswaran Saravanan, Sanjukta Patra (2013). Design of lead peptide drugs from mushroom targeting cysteine proteases. Medicinal Chemistry Research 22(4), 2038-2049.
  16. Sumit K Singh, Anurag S Rathore (2014). Clinical and Non-Clinical aspects of biosimilar development .BioQuality 19(9-10),2-6 
  17.  Douglas Nmagu, Sumit K. Singh, Kelvin H. Lee. Creation of monoclonal antibody expressing CHO cell lines grown with sodium butyrate and characterization of resulting antibody glycosylation. Methods in Enzymology (2021).
  18. Anurag S Rathore, Sumit K Singh, J Kumar, G Kapoor. (2018) Implementation of QbD for Manufacturing of Biologics—Has It Met the Expectations? Biopharmaceutical Processing, 1051-1073.
  19. Anurag S Rathore, Sumit K Singh (2017). Chemometrics Applications in Process Chromatography.  Preparative Chromatography for Separation of Proteins, 479- 500.
  20. Anurag S Rathore, Sumit K Singh (2016). Production of protein therapeutics in the quality by design (QbD) paradigm. Protein Therapeutics, 41-67.
  21. Anurag S Rathore, Sumit K Singh, Neh Nupur, Gunjan Narula. (2016). Role of proteomics in characterization of biosimilar products. Biomarker Discovery in the Developing World: Dissecting the Pipeline for Meeting the Challenges, 83-97.

List of Important Presentations

After Joining IIT-BHU

  1. Sumit K. Singh,  Concepts in liquid chromatography: Instrumental aspects,  Hands-on Training Programme on Bioprocessing, June 2022, IIT Delhi, India  [INVITED TALK]
  2. Sumit K. Singh, Controlling Critical Quality Attributes of Biopharmaceutical products, Center of Excellence for Biopharmaceutical Technology Course Series, April 2022, IIT Delhi, India  [INVITED TALK]
  3. Sumit K Singh, Case studies of mass spectrometry-based characterization of biopharmaceutical products, Center of Excellence for Biopharmaceutical Technology Course Series, December, 2021, IIT Delhi, India  [INVITED TALK]
  4. Sumit K Singh, Characterization of primary structure and post-translational modifications of biopharmaceuticals, Center of Excellence for Biopharmaceutical Technology Course Series, December, 2021, IIT Delhi, India  [INVITED TALK]
  5. Sumit K Singh, Multivariate Data Analysis for Biotech Processes:  Concepts and Case Studies, FDP on Recent Developments in the field of Computational Biology, Institute of Engineering and Management, November 2021, Kolkata, India [INVITED TALK]

Before joining IIT-BHU

  1. Sumit K Singh, Kelvin H Lee. Monoclonal antibody glycan analysis using mass spectrometry.  American Chemical Society National Meeting, August 2020, Virtual.
  2. Sumit K Singh, D Kumar, AS Rathore. Activity loss due to methionine oxidation in GCSF: Mechanism and control, Gordon Research Conference on Biotherapeutics and Vaccine Development, January 2019, Galveston, Texas[INVITED TALK]. 
  3. Sumit K Singh, D Kumar, DK Yadav, AS Rathore. Identification of critical quality attributes of biotherapeutics: GCSF as a case study.  American Chemical Society National Meeting, April 2017, San Francisco
  4. Sumit K Singh, AS Rathore. Fermentanomics: Relating quality attributes of a monoclonal antibody to cell culture process variables and raw materials using multivariate data analysis. American Chemical Society National Meeting, April 2017, San Francisco. 
  5. Sumit K. Singh, Anurag S. Rathore, Multivariate data analysis for biotech processes: Industrial case studies, American Chemical Society National Meeting, April 2017, San Francisco
  6. Sumit K Singh, AS Rathore. Modulation of granulocyte colony stimulating factor conformation and receptor binding by methionine oxidation, Bioprocessing India Conference 2018, IIT Delhi
  7. Sumit K Singh, D Kumar, AS Rathore, Investigating the effect of oxidation of granulocyte colony stimulating factor on biological activity using experimental and computational approach, American Chemical Society National Meeting, April 2017, San Francisco
  8. Sumit K Singh, AS Rathore. Host Cell Protein Analysis by HPLC/CE-ESI-TOF-MS. Targeted Proteomics workshop and International Symposium, December 2015, IIT Mumbai [INVITED TALK]. 

List of Important Posters

  1. Sumit K Singh, AS Rathore. Modulation of granulocyte colony stimulating factor conformation and receptor binding by methionine oxidation. Bioprocessing India Conference, December 2018, IIT Delhi.
  2. Sumit K Singh, D Kumar, AS Rathore. Investigating the effect of oxidation of granulocyte colony stimulating factor on biological activity using experimental and computational approach. American Chemical Society National Meeting, April 2017, San Francisco.
  3. Sumit K Singh, D Chakravorty, S Patra. In silico prediction of mechanism of action of cysteine protease inhibitors from mushroom. Annual conference of Association of Microbiologist of India, December 2010, BIT Mesra.

 

Selected Awards

  1. Invited Speaker, Gordon Research Conference on Vaccine and Biotherapeutic Development, Galveston, Texas, USA (2019)
  2. Young Scientist in Bioprocessing Award by Bioprocessing India Conference 2018, India.
  3. Research Excellence Travel Award, IIT Delhi, 2018.
  4. ACS BIOT Travel subsidy Award, American Chemical Society, 2017.
  5. International Travel Award, DBT, Government of India, 2017.
  6. Best Oral Presentation Award, Targeted Proteomics workshop and International Symposium, IIT Mumbai, 2015.
  7. Adani Education Scholarship Award, Adani Group, 2013-2014.
  8. Graduate Aptitude Test in Engineering, Biotechnology, 2011.
  9. Best Poster Presentation Award, NIT Raipur, 2010.

Membership of Societies

  1. Bioprocessing Society of India (Life Member)
  2. Member of American Chemical Society (BIOT Division)
  3. Member of American Society for Mass Spectrometry (ASMS)

Peer-reviewer for Journals (ad-hoc basis)

1. Journal of Chromatography B
2. Bioanalysis
3. Biotechnology Progress
4. Engineering in Life Sciences
5. Biotechnology and Bioengineering
6. Scientific Reports
7. Oxidative Medicine and Cellular Longevity
8. Heliyon (Cell Press)
9. Current Research in Biotechnology

Laboratory of Engineered Therapeutics

Our research interest is to develop the structure-function relationship of various post-translational modifications (PTMs) in proteins and evaluate their role in the context of various human diseases. The research program in our group will integrate concepts from analytical chemistry, proteomics, immunology, and structural biology to gain insights into the following broad subtopics: 

Currently, the broad aims of research in our group are: 

  • Understanding how glycans impact protein and viral function?

  • How they are structured?

  • How can they be engineered for therapeutic application?

Tools we employ to accomplish our research objectives: 

 

  • Liquid Chromatography-Mass Spectrometry (LC-MS)

  • Molecular Dynamics (MD) Simulation 

  • Cell Culture/Animal Experiments

Research Collaborations

Academia

1. Bioseparations &  Bioprocessing Lab,  IIT Delhi 
2.  Chemical Engineering & Process development group, National Chemical Laboratory (NCL), Pune
  

Industry

1. Symphony Tech Biologics Pvt Ltd, Pune
2. Edna Biolabs, New Delhi

 

 

Odd Sem 2021-22

1. Protein Engineering (BC 536) 

Even Sem 2021-22

1. Bioprocess Calculations (BC 212)
2. Structural Biology (BC 333)
3. Molecular Biology and Genetic Engineering Lab (BC 332)

 

Sl. No Student Fellowship Degree Year Topic Status
1. Santanu Singh MHRD Ph.D. January 2022 Characterization of Post-translational modifications (PTMs) of bio-therapeutics Ongoing
2. Kajal Kachhawaha DBT-JRF Ph.D. January 2022 Cloning, Expression, and Purification of Antibody-Drug Conjugates (ADCs) Ongoing
3. Sahil Khan MHRD M.Tech February 2022 Modeling of glycosylation in CHO Ongoing

Others: 
1. Aryaman Joshi (B.Tech, Chemical Engineering, IIT BHU) - Working on understanding Fc sialyation in IgG using MD simulations ( Dec 2021 onwards)

 

  •  Member, Department Undergraduate Committee (DUGC) : 2021-22
  •  Member,  Department Postgraduate Committee (DPGC) : 2021-22
  • Member, Alumni Affairs Committee, SBCE : 2021-22
  • Departmental Faculty Representative, Advisory committee of Training and Placement Cell, IIT BHU (2022-23) 
  • External Subject Expert- SERB-SRG Grant (M-14-0595)
  • Member, General Body, Bioprocessing India Society.
  • Member, Working Committee, Bioprocessing India Society. 
Sl No.                             Project Awarding Agency Role Year Status
  1.  
Structural and Functional Characterization Studies to Establish Comparability of Biosimilars  
  IIT BHU
 
  PI
 
  2021-22
  Ongoing
 

We are looking for highly motivated Post-doctoral fellows, PhD students, and Project fellows to join our group and work on some exciting projects on understanding and engineering Critical Quality Attributes (CQAs) of biopharmaceuticals. Prior experience  in Proteomics/ glycoproteomics,  Chromatographic Process Development, and Molecular Dynamic (MD) Simulations  of proteins is desirable, but not essential. Interested candidates can contact the principal investigator (sumit.bce@iitbhu.ac.in) following the institute norms. 

We are also happy to consider hosting undergraduate/Postgraduate students for short term research projects (minimum 6 months).