* Corresponding author

Publications after joining IIT BHU

Books

1. Sumit K. Singh et al. (Eds.) (2023). Protein Biomarkers: Discovery and Applications in Clinical Diagnostics. Publisher: Springer, Singapore.

Journal Articles/Book Chapters

1. Santanu Singh, Rohan Shah, Sumit K. Singh* (2024) Comparative analysis of shotgun and targeted proteomics: Insights for biomarker research.Protein Biomarkers: Discovery and Application in clinical diagnostics (Accepted)
2. Kajal Kachhawaha, Rohan Shah, Sumit K. Singh* (2024) Clinical sample considerations in proteomics-based biomarker studies: advancing precision medicine. Protein Biomarkers: Discovery and Application in clinical diagnostics (Accepted)
3. Khyati Joshi, Rohan Shah,Sumit K. Singh* (2024). Experimental design in proteomic mass spectrometry studies for biomarker discovery. Protein Biomarkers: Discovery and application in clinical diagnostics (Accepted)
4. Santanu Singh, Khyati Joshi, Kajal Kacchawaha, Niharika G. Jha, Sahil Khan, Sumit K. Singh* (2023). Non-clinical safety and efficacy assessment of emerging next-generation therapeutics.Biotechnology and Genetic Engineering Reviews (Accepted)
5. Sahil Khan, Aryaman Joshi, Sumit K. Singh*, Modeling of N-linked glycosylation: Advances and Challenges in predicting glycan structures during biomanufacturing. Preprint: Doi: 1022541/au.168382757.72018049/v1
6. Niharika G. Jha, Daphika S. Dkhar, Sumit K.Singh, Shweta J. Malode,Nagaraj P. Shetty, Pranjal Chandra (2023) Engineered biosensors for diagnosing multi-drug resistance in microbial and malignant cells. Biosensors (Accepted)
7. Aryaman Joshi, Timir Tripathi, Sumit K.Singh, Aditya K.Padhi(2022).Computational approaches to develop engineered therapeutics against SARS-CoV2. ACS Biochemistry (Accepted).
8. Kajal Kachhawaha, Santanu Singh, Khyati Joshi, Priyanka Nain, Sumit K. Singh* (2022). Bioprocessing of recombinant proteins from Escherichia coli Inclusion bodies: Insights from structure-function relationship for novel applications. Journal of Preparative Biochemistry and Biotechnology (Accepted) IF: 3.12 
    
9. Sumit K. Singh, Deepak Kumar, Saurabh Nagpal, Sunil K. Dubey, Anurag S. Rathore (2022).  A charge variant of bevacizumab offers enhanced FcRn-dependent pharmacokinetic half-life and efficacy. Pharmaceutical Research (In press). IF: 4.91
    
10. Sumit K Singh, Kelvin H Lee (2021) Characterization of monoclonal antibody glycan heterogeneity using hydrophilic interaction liquid chromatography-mass spectrometry. Frontiers in Bioengineering and Biotechnology (In press) DOI : 10.3389/fbioe.2021.805788 IF: 6.06
     
11. Rozaleen Dash, Sumit K Singh, Narendra Chirmule, Anurag S Rathore (2021). Assessment of functional characterization and comparability of biotherapeutics: A review. The AAPS Journal (In press) DOI: 10.1208/s12248-021-00671-0. IF: 4.46

     Publications before joining IIT-BHU

12. Sumit K Singh, Deepak Kumar, Anurag S Rathore (2020). Understanding Oxidation Propensity in GCSF and Assessment of its Safety and Efficacy. Pharmaceutical Research, 37, (207). IF: 4.91
13. Sumit K Singh, A Mishra, N Chirmule, G Goel, Anurag S Rathore (2020) Modulation of granulocyte colony stimulating factor conformation and receptor binding by methionine oxidation. Proteins: Structure, Function and Bioinformatics, 1-13. IF: 4.08
14. Sumit K Singh, D Kumar, Anurag S Rathore (2021) LC-MS based case-by-case analysis of the impact of acidic and basic charge variants of bevacizumab on stability and biological activity. Scientific Reports, 11(1), 1-15. IF: 4.99
15. Avinash Mishra, Rucha Patil, Sumit K Singh, Priyanka Dalal, Anurag S. Rathore (2020) Mechanistic explanation of structural and functional changes induced by methionine mutation in G-CSF protein. Current Research in Biotechnology, 2, 37-44. IF: 5.19
16. Sumit K Singh, Avinash Mishra, Divyanshi Yadav, Niharika Budholiya, Anurag S Rathore (2019) Understanding the mechanism of copurification of “difficult to remove” host cell proteins in rituximab biosimilar products. Biotechnology Progress, e2936. IF: 2.90
17. Sumit K Singh, G Goel, Anurag S Rathore. (2019). A novel approach for protein identification from complex cell proteome using modified peptide mass fingerprinting algorithm. Electrophoresis,40, (23-24), 3062-3073. IF: 3.59
18. Anurag S Rathore, Dinesh K Yadav, Shyam S Pandey, Sumit K Singh, Deepak Kumar (2018). Preclinical Evaluation of Product Related Impurities and Variants.  Biopharm International, 31 (2), 26-31.
19. Sumit K Singh, Deepak Kumar, Anurag S Rathore. (2017). Determination of critical quality attributes for a biotherapeutic in the QbD paradigm: GCSF as a case study. The AAPS Journal, 19(6), 1826-1841. IF: 4.46
20. Neh Nupur, Sumit K Singh, Gunjan Narula, Anurag S Rathore (2017). Assessing analytical comparability of biosimilars: GCSF as a case study. Journal of Chromatography B, 1032, 165-171. IF :3.38
21. Sumit K Singh, Vishwanath Hebbi, Anurag S Rathore, Ira S Krull (2016). Practical Considerations of Capillary Electrophoresis–Mass Spectrometry for Analysis of Biotherapeutics. LC-GC North America, 34(9), 720-728.
22. Gunjan Narula, Vishwanath Hebbi, Sumit K Singh, Anurag S Rathore. (2016). Glycosylation in mAb therapeutic products: analytical characterization and impact of process. LC-GC Europe, 29(7), 385-392.
23. Sumit K Singh, G Narula, AS Rathore (2016). Should charge variants of monoclonal antibody therapeutics be considered critical quality attributes? Electrophoresis, 37(17-18), 2338-2346.
24. Sumit K Singh, Anurag S Rathore (2015). Use of multivariate data analysis in bioprocessing.  Biopharm International, 28(6), 26-31.
25. Anurag S. Rathore, Sumit K Singh, Mili Pathak, Erik K. Read, Kurt A. Brorson, Cyrus D. Agarabi, Mansoor Khan (2015) Fermentanomics: Relating quality attributes of a monoclonal antibody to cell culture process variables and raw materials using multivariate data analysis, Biotechnology Progress, 31 (6), 1586-1599.
26. Debamitra Chakravorty, Sumit K. Singh, Parameswaran Saravanan, Sanjukta Patra (2013). Design of lead peptide drugs from mushroom targeting cysteine proteases. Medicinal Chemistry Research 22(4), 2038-2049.
27. Sumit K Singh, Anurag S Rathore (2014). Clinical and Non-Clinical aspects of biosimilar development .BioQuality 19(9-10),2-6 
28.  ​Douglas Nmagu, Sumit K. Singh, Kelvin H. Lee. Creation of monoclonal antibody expressing CHO cell lines grown with sodium butyrate and characterization of resulting antibody glycosylation. Methods in Enzymology (2021).
    
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29. Anurag S Rathore, Sumit K Singh, J Kumar, G Kapoor. (2018) Implementation of QbD for Manufacturing of Biologics—Has It Met the Expectations? Biopharmaceutical Processing, 1051-1073.
    
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30. Anurag S Rathore, Sumit K Singh (2017). Chemometrics Applications in Process Chromatography.  Preparative Chromatography for Separation of Proteins, 479- 500.
31. Anurag S Rathore, Sumit K Singh (2016). Production of protein therapeutics in the quality by design (QbD) paradigm. Protein Therapeutics, 41-67.
    
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32. Anurag S Rathore, Sumit K Singh, Neh Nupur, Gunjan Narula. (2016). Role of proteomics in characterization of biosimilar products. Biomarker Discovery in the Developing World: Dissecting the Pipeline for Meeting the Challenges, 83-97.
    
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List of Important Presentations/meetings

After Joining IIT-BHU

1. Sumit K. Singh. Clinical Assay development: Challenges for translation. Advances in Proteomic Technologies Conference, IIT Mumbai (February 2024) [coordinator/moderator of panel discussion]
2. Sumit K. Singh. Mapping mAb critical quality attribute: Modeling glycosylation from mass spectrometry data. Advances in Proteomic Technologies conference, IIT Mumbai (February 2024). [ECR Talk]
3. Sumit K. Singh, Characterization of proteins and polymers using mass spectrometry and other analytical methods, Department of polymer and process engineering,  IIT Roorkee (December 2023)- [INVITED TALK]
4. Sumit K.Singh, PTM  characterization of biotherapeutics using native mass spectrometry, CoE CBT Course Series, 2023, IIT Delhi  (December 2023) [INVITED TALK]
5. Sumit K. Singh, Introduction to native mass spectrometry, CoE CBT Course series, 2023, IIT Delhi (December 2023) [Keynote Lecture]
6. JV Reddy, Sumit K Singh, T Leibiger, E Papoutsakis, M Ierapetritou. Insights into the effect of bioreactor pH on Chinese Hamster Ovary cell metabolism and site-specific N-linked glycosylation of VRC01 mAb, AIChE Annual Meeting, Orlando, FL (November 2023)
7. Sumit K. Singh, Exploring high-resolution mass spectrometry for biopharma applications, SCIEX Webinar (online) [Invited talk] (September 2023)
8. Sumit K. Singh, Modeling N-Glycosylation in Chinese Hamster Ovary (CHO) cells expressing VRC-01 antibody. 9th Metabolic Pathway Analysis Conference, Seoul, South Korea (July 2023) [Lightning Talk]
9. Sumit K.Singh, MolDev techtalk, Molecular devices, Bangalore
10. Sumit K.Singh, Biopharma Workshop, CoE SCIEX India, Bangalore, 2023
11. Sumit K. Singh, Technqiues to study proteins to proteome, Hands-on-training on biological and molecular techniques, Department of Zoology, MMV, Banaras Hindu University, March 2023 [Invited talk]
12. Sumit K. Singh, Activity loss due to methionine oxidation in GCSF: mechanism and control, 8th International Bioprocessing India conference,  December 2022, CSIR-NCL Pune [Invited Talk]
13. Sumit K. Singh, Characterization of primary structure and post-translational modifications using multi-attribute monitoring workflows, Center of Biopharmaceutical Technology Course Series, December 2022,  IIT Delhi, India [Invited Talk]
14. Sumit K. Singh, Characterization of biopharmaceuticals using peptide mapping workflows, Center of Biopharmaceutical Technology Course Series, December 2022, IIT Delhi, India [Invited Talk]
15. Sumit K. Singh. Theory and practice of native mass spectrometry, Center of Excellence for Biopharmaceutical Technology Course Series, December 2022, IIT Delhi, India [Invited Talk]
16. Sumit K. Singh, Bioprocessing of mAbs:  producing safe and efficacious biologics. Expanding Horizons of Downstream Processing, July 2022, Kalasaligam Academy of Research and Education, Krishnankoil, Tamil Nadu, India [INVITED TALK]
17. Sumit K. Singh,  Concepts in liquid chromatography: Instrumental aspects,  Hands-on Training Programme on Bioprocessing, June 2022, IIT Delhi, India  [INVITED TALK]
18. Sumit K. Singh, Controlling Critical Quality Attributes of Biopharmaceutical products, Center of Excellence for Biopharmaceutical Technology Course Series, April 2022, IIT Delhi, India  [INVITED TALK]
19. Sumit K Singh, Case studies of mass spectrometry-based characterization of biopharmaceutical products, Center of Excellence for Biopharmaceutical Technology Course Series, December, 2021, IIT Delhi, India  [INVITED TALK]
20. Sumit K Singh, Characterization of primary structure and post-translational modifications of biopharmaceuticals, Center of Excellence for Biopharmaceutical Technology Course Series, December, 2021, IIT Delhi, India  [INVITED TALK]
21. Sumit K Singh, Multivariate Data Analysis for Biotech Processes:  Concepts and Case Studies, FDP on Recent Developments in the field of Computational Biology, Institute of Engineering and Management, November 2021, Kolkata, India [INVITED TALK]

Before joining IIT-BHU

1. Sumit K Singh, Kelvin H Lee. Monoclonal antibody glycan analysis using mass spectrometry.  American Chemical Society National Meeting, August 2020, Virtual.
2. Sumit K Singh, D Kumar, AS Rathore. Activity loss due to methionine oxidation in GCSF: Mechanism and control, Gordon Research Conference on Biotherapeutics and Vaccine Development, January 2019, Galveston, Texas[INVITED TALK]. 
3. Sumit K Singh, D Kumar, DK Yadav, AS Rathore. Identification of critical quality attributes of biotherapeutics: GCSF as a case study.  American Chemical Society National Meeting, April 2017, San Francisco
4. Sumit K Singh, AS Rathore. Fermentanomics: Relating quality attributes of a monoclonal antibody to cell culture process variables and raw materials using multivariate data analysis. American Chemical Society National Meeting, April 2017, San Francisco. 
5. Sumit K. Singh, Anurag S. Rathore, Multivariate data analysis for biotech processes: Industrial case studies, American Chemical Society National Meeting, April 2017, San Francisco
6. Sumit K Singh, AS Rathore. Modulation of granulocyte colony stimulating factor conformation and receptor binding by methionine oxidation, Bioprocessing India Conference 2018, IIT Delhi
7. Sumit K Singh, D Kumar, AS Rathore, Investigating the effect of oxidation of granulocyte colony stimulating factor on biological activity using experimental and computational approach, American Chemical Society National Meeting, April 2017, San Francisco
8. Sumit K Singh, AS Rathore. Host Cell Protein Analysis by HPLC/CE-ESI-TOF-MS. Targeted Proteomics workshop and International Symposium, December 2015, IIT Mumbai [INVITED TALK]. 

List of Important Posters

1. Sumit K Singh, AS Rathore. Modulation of granulocyte colony stimulating factor conformation and receptor binding by methionine oxidation. Bioprocessing India Conference, December 2018, IIT Delhi.
2. Sumit K Singh, D Kumar, AS Rathore. Investigating the effect of oxidation of granulocyte colony stimulating factor on biological activity using experimental and computational approach. American Chemical Society National Meeting, April 2017, San Francisco.
3. Sumit K Singh, D Chakravorty, S Patra. In silico prediction of mechanism of action of cysteine protease inhibitors from mushroom. Annual conference of Association of Microbiologist of India, December 2010, BIT Mesra.

Selected Awards

1. International Travel grant- SERB under Young Scientist Scheme ( 2023)
2. Key Opinion Leader (KOL)- Mass Spectrometry/Biopharma (SCIEX, India)
3. Included in the Invited list to attend the 3rd meeting of "Human Glycome Project" at Split, Croatia (2022)- only participant from India.
4. Invited Speaker, 1st Gordon Research Conference on Vaccine and Biotherapeutic Development, Galveston, Texas, USA (2019)
5. Young Scientist in Bioprocessing Award by Bioprocessing India Conference 2018, India.
6. Research Excellence Travel Award, IIT Delhi, 2018.
7. ACS BIOT Travel subsidy Award, American Chemical Society, 2017.
8. International Travel Award, DBT, Government of India, 2017.
9. Best Oral Presentation Award, Targeted Proteomics workshop and International Symposium, IIT Mumbai, 2015.
10. Adani Education Scholarship Award, Adani Group, 2013-2014.
11. Graduate Aptitude Test in Engineering, Biotechnology, 2011.
12. Best Poster Presentation Award, NIT Raipur, 2010.

Membership of Societies

1. Bioprocessing Society of India (Life Member)
2. Member of American Chemical Society (BIOT Division)
3. Member of American Society for Mass Spectrometry (ASMS)
4. Member of  The Antibody Society

Editorial Assignments

1.  Guest Associate Editor, Frontiers in Analytical Science : Submissions Deadline ( 31 May 2024)

Peer-reviewer for Journals (ad-hoc basis)

1. Journal of Chromatography B
2. Bioanalysis
3. Biotechnology Progress
4. Engineering in Life Sciences
5. Biotechnology and Bioengineering
6. Scientific Reports
7. Oxidative Medicine and Cellular Longevity
8. Heliyon (Cell Press)
9. Current Research in Biotechnology
10. Analytical and Bioanalytical  Chemistry
11.  Int. J. of Bioinformatics Research and Applications
12. Journal of Scientific and Industrial Research
13. Environment, Development, and Sustainability

14. Reaction Chemistry and Engineering 

15. International Journal of Biological Macromolecules

16. Food and Bioproducts Processing

17. Journal of Travel Medicine (Oxford Press)

Project 1: Understanding the Influence of Glycosylation on Microglial Cells and Alzheimer's Disease

Alzheimer's Disease (AD) poses a significant global health challenge. In this project, we are delving into the intricate world of glycosylation and its impact on microglial cells, which play a pivotal role in neuroinflammation and AD progression. By deciphering the glycosylation patterns that modulate the structure and function of microglial cells, we aim to uncover novel targets for the development of innovative drugs to tackle this debilitating disorder.

Key Objectives : 

- Investigate the glycosylation patterns of microglial cells in AD.
- Uncover how glycosylation influences microglial cell structure and function.
- Identify potential drug targets for AD therapy based on glycosylation insights.

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Project 2: Harnessing Glycoengineering for Antibody-Drug Conjugate Development

Antibody-Drug Conjugates (ADCs) have revolutionized targeted cancer therapy. In this project, we're taking ADCs to the next level by targeting sialic acids at the monoclonal antibody's (mAb) Fc region. By introducing terminal sialylation through glycoengineering, we're aiming to create a platform for precise drug conjugation. This innovative approach addresses the challenge of Fc sialylation in recombinant mAbs and paves the way for enhanced ADC efficacy.

Key Objectives:

- Develop glycoengineered mAbs with terminal sialylation for optimal drug conjugation.
- Investigate the site-specific drug conjugation potential at Fc region sialic acids.
- Elevate the effectiveness of ADCs by harnessing glycoengineering techniques.

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Project 3: Biopharmaceutical Processing and Characterization

1. Structure-Function Evaluation of Deamidation in Monoclonal Antibodies (mAbs)

• In Collaboration with Sciex

Monoclonal antibodies (mAbs) are indispensable therapeutic agents, but post-translational modifications like deamidation can impact their efficacy and stability. In collaboration with Sciex, we employ cutting-edge mass spectrometry techniques to elucidate the structural consequences of deamidation in mAbs, ultimately enhancing our ability to design and optimize these life-saving biotherapeutics.

2. Modeling of Chinese Hamster Ovary (CHO) Glycosylation

• In Collaboration with SymphonyTech Biologics

Chinese Hamster Ovary (CHO) cells are commonly used in biopharmaceutical production. Our collaboration with SymphonyTech Biologics focuses on developing advanced models for CHO cell glycosylation. Mass spectrometry plays a pivotal role in characterizing and predicting glycan profiles, offering valuable insights into bioprocessing and glycoengineering strategies.

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Our Toolbox for Research Advancement

• Liquid Chromatography-Mass Spectrometry (LC-MS)

• Molecular Dynamics (MD) Simulation 

• Cell Culture/Animal Experiments

• Biophysical Characterization tools (FTIR, Fluorescence, Circular Dichroism, Flow cytometry) 

Research Collaborations

• Academia

          1. Bioseparations &  Bioprocessing Lab,  IIT Delhi 
          2.  Chemical Engineering & Process development group, National Chemical Laboratory (NCL), Pune 

          3. Vaccine and Immunotherapy Center, The Wistar Institute, USA
 

• Industry

        1. Symphony Tech Biologics Pvt Ltd, Pune 

        2. SCIEX 

Odd Sem 2021-22

1. Protein Engineering (BC 536) 

Even Sem 2021-22

1. Bioprocess Calculations (BC 212)
2. Structural Biology (BC 333)
3. Molecular Biology and Genetic Engineering Lab (BC 332)

Odd Sem 2022-23

1. Microbial Engineering (BC 311)
2. Protein Engineering( BC 536)
3. Enzyme Engineering Lab (BC 323)

Even Sem 2022-23

1. Bioprocess Calculations (BC 212)
2. Structural Biology (BC 333)

Odd Sem 2023-24

1. Protein Engineering (BC 536)

2. Microbial Engineering (BC 311)

Alumni

• External Expert, Ph.D. evaluation, IIT Madras (2023, 2024)
• Member, Department Undergraduate Committee (DUGC) : 2021-22 & 2022-23
• Member,  Department Postgraduate Committee (DPGC) : 2021-22; 2023-24
• Member, Ph.D. Admission Committee 2021 (SBCE, IIT BHU)
• Member, M.Tech. Admission Committee 2021 (SBCE, IIT BHU)
• Member,  Alumni Affairs Committee, SBCE: 2021-22
• Member of Advisory Committee,Training and Placement Cell, IIT BHU (2022-23) 
• Member, Selection Committee,  SERB-DST sponsored project at Dept. of Zoology, MMV, BHU (Project Code: M-14-0595)
• External expert, Ph.D. evaluation, CSIR National Chemical Laboratory (NCL) Pune (2022)
• Member, Working Committee, Bioprocessing India Society. 
• Member Organizing Committee, I-DAPT Health-Tech Hackathon 2022, IIT BHU
• Counting officer, Election duty for student's parliament, 2022, IIT BHU
• Session Chair, 8th International Bioprocessing India conference 2022, NCL Pune
• Session Moderator, Poster presentation, 8th International Bioprocessing India conference 2022, NCL Pune
• Co-organizing secretary, Short term course on Computer-aided drug design and structural bioinformatics (CADDSB -2023), March 2023
• Deputy Presiding Officer, Students parliamentary election, 2023, IIT BHU
• Member, Coordination team, SATURNALIA 2023, IIT BHU, September 2023. 

We are looking for highly motivated post-doctoral fellows, Ph.D. students, and Project fellows to join our group and work on exciting projects to understand the role of glycans in various diseases. We also develop analytical methods for the in-depth characterization of biopharmaceuticals.  Prior experience in protein expression, cloning, purification, and proteomics/ glycoproteomics is desirable but not essential. Interested candidates can contact the principal investigator (sumit.bce@iitbhu.ac.in) following the institute norms.