Selected Awards

1. International Travel grant- SERB under Young Scientist Scheme ( 2023)
2. Key Opinion Leader (KOL)- Mass Spectrometry/Biopharma (SCIEX, India)
3. Included in the Invited list to attend the 3rd meeting of "Human Glycome Project" at Split, Croatia (2022)- only participant from India.
4. Invited Speaker, 1st Gordon Research Conference on Vaccine and Biotherapeutic Development, Galveston, Texas, USA (2019)
5. Young Scientist in Bioprocessing Award by Bioprocessing India Conference 2018, India.
6. Research Excellence Travel Award, IIT Delhi, 2018.
7. ACS BIOT Travel subsidy Award, American Chemical Society, 2017.
8. International Travel Award, DBT, Government of India, 2017.
9. Best Oral Presentation Award, Targeted Proteomics workshop and International Symposium, IIT Mumbai, 2015.
10. Adani Education Scholarship Award, Adani Group, 2013-2014.
11. Graduate Aptitude Test in Engineering, Biotechnology, 2011.
12. Best Poster Presentation Award, NIT Raipur, 2010.

Membership of Societies

1. Bioprocessing Society of India (Life Member)
2. Member of American Chemical Society (BIOT Division)
3. Member of American Society for Mass Spectrometry (ASMS)
4. Member of  The Antibody Society

Editorial Assignments

1.  Guest Associate Editor, Frontiers in Analytical Science : Submissions Deadline ( 31 May 2024)

Peer-reviewer for Journals (ad-hoc basis)

1. Journal of Chromatography B
2. Bioanalysis
3. Biotechnology Progress
4. Engineering in Life Sciences
5. Biotechnology and Bioengineering
6. Scientific Reports
7. Oxidative Medicine and Cellular Longevity
8. Heliyon (Cell Press)
9. Current Research in Biotechnology
10. Analytical and Bioanalytical  Chemistry
11.  Int. J. of Bioinformatics Research and Applications
12. Journal of Scientific and Industrial Research
13. Environment, Development, and Sustainability

14. Reaction Chemistry and Engineering 

15. International Journal of Biological Macromolecules

16. Food and Bioproducts Processing

17. Journal of Travel Medicine (Oxford Press)

18. Biochemical and Biophysical Research Communications

19. Journal of Biochemical and Molecular Toxicology

20. Industrial Crops and Products

Project 1: Understanding the Influence of Glycosylation on Microglial Cells and Alzheimer's Disease (Shantanu Singh)

Project 2: Harnessing Glycoengineering for Antibody-Drug Conjugate Development (Kajal Kachhwaha)

Project 3: Glycoengineering of Exosomes for therapeutic applications (Jyoti Verma)

Project 4:  Silk-based biomaterials for cosmeceutical applications (Manisha Mishra)

Industrial Collaborative Projects: 

Project 5: Development of LC-MS workflows for mAb characterization (Shantanu Singh, Kajal Kachhwaha, Jyoti Verma)-  with  SCIEX India Ltd

Project 6: Modeling of Chinese Hamster Ovary (CHO) Glycosylation (Pranav Chopra)-  with Symphonytech Biologics Private Ltd. 

1. Protein Engineering (BC 536) - Odd Sem 2021-22, Odd Sem 2022-23, Odd Sem 2023-24

2. Bioprocess Calculations (BC 212)-  Even Sem 2021-22, Even Sem 2022-23, Even Sem 2023-24

3. Structural Biology (BC 333) - Even Sem 2021-22, Even Sem 2022-23, Even Sem 2023-24

4. Molecular Biology and Genetic Engineering Lab (BC 332) -Even Sem 2021-22

5. Microbial Engineering (BC 311) - Odd Sem 2022-23, Odd Sem 2023-24

6. Enzyme Engineering Lab (BC 323)- Odd Sem 2022-23

• Member, Institute Level Standing Committee for the condemnation of Biomedical Engineering, Metallurgical Engineering, and Proctor Office.  
• Chairman: PhD Admission BCE 2024-2025 Even Semester
• Coordinator for the Intellectual Property Rights (IPR) Awareness Program under the National Intellectual Property Awareness Mission 2.0 (NIPAM), overseen by the Department for Promotion of Industry and Internal Trade (DPIIT), Ministry of Commerce & Industry, Government of India.
• Convener,  Departmental Postgraduate Committee (DPGC)- (2024- )
• Member, Organizing Committee "Research and Innovation Day / Teachers Day" celebration, 5th September; 2024
• Counting Officer, Election duty for student's parliament, 2024, IIT BHU
• Member, Department Purchase Committee ( 2024-25)
• External Examiner for setting question paper for NIPER JEE
• External Expert, Ph.D. evaluation, IIT Madras (2023, 2024)
• Member, Department Undergraduate Committee (DUGC) : 2021-22 & 2022-23
• Member,  Department Postgraduate Committee (DPGC) : 2021-22; 2023-24
• Member, Ph.D. Admission Committee 2021 (SBCE, IIT BHU)
• Member, M.Tech. Admission Committee 2021 (SBCE, IIT BHU)
• Member,  Alumni Affairs Committee, SBCE: 2021-22
• Member of Advisory Committee,Training and Placement Cell, IIT BHU (2022-) 
• Member, Selection Committee,  SERB-DST sponsored project at Dept. of Zoology, MMV, BHU (Project Code: M-14-0595)
• External expert, Ph.D. evaluation, CSIR National Chemical Laboratory (NCL) Pune (2022)
• Member, Working Committee, Bioprocessing India Society. 
• Member Organizing Committee, I-DAPT Health-Tech Hackathon 2022, IIT BHU
• Counting officer, Election duty for student's parliament, 2022, IIT BHU
• Session Chair, 8th International Bioprocessing India conference 2022, NCL Pune
• Session Moderator, Poster presentation, 8th International Bioprocessing India conference 2022, NCL Pune
• Co-organizing secretary, Short term course on Computer-aided drug design and structural bioinformatics (CADDSB -2023), March 2023
• Deputy Presiding Officer, Students parliamentary election, 2023, IIT BHU
• Member, Coordination team, SATURNALIA 2023, IIT BHU, September 2023. 

We are looking for highly motivated post-doctoral fellows, Ph.D. students, and Project fellows to join our group and work on exciting projects to understand the role of glycans in various diseases. We also develop analytical methods for the in-depth characterization of biopharmaceuticals.  Prior experience in protein expression, cloning, purification, and proteomics/ glycoproteomics is desirable but not essential. Interested candidates can contact the principal investigator (sumit.bce@iitbhu.ac.in) following the institute norms. 

* Corresponding author

Publications after joining IIT BHU

Patents: 

1. Sumit K. Singh. Silk Fibroin Protein Formulation for Enhanced Hair Cuticle Orientation and Color Retention During Dyeing. (Status: Filing in process)

2. Sumit K. Singh. Kaunch Seed Extract-Based Natural Hair Color for Dyeing Applications. (Status: Filing in process)

3. Sumit K. Singh. Composition and Method for Applying Natural Hair Color Formulation Utilizing Silk Fibroin and Kaunch Seed Extract (Status: Filing in process)

4. Pranjal Chandra, Indrani Nandi, Rohini Kumari, Sumit Kumar Singh, Kajal Kachhawaha.  An enzyme mimicking nanosensor and a method of fabrication thereof (Application No.: 202411032581)  (Status: FILED) 

Books:

1. Sumit K. Singh et al. (Eds.) (2024). Protein Biomarkers: Discovery and Applications in Clinical Diagnostics. Publisher: Springer, Singapore. (ISBN: 978-9819750443)

Journal Articles & Book Chapters : 

1. Shubhangi, Rohini Kumari, Kajal Kachhawaha, Sumit K. Singh, Sanjay Rai, Pranjal Chandra (2024). Pristine NiMOF sandwiched between 1D and 3D engineered Au-particles and dendritic platform for ultra-swift folic acid sensing in cellular microenviornment. ACS Analytical Chemistry (Accepted)
2. Jayanth Venkatarama Reddy, Sumit K. Singh, Thomas Leibiger, Kelvin H Lee, Marianthi Ierapetritou, Eleftherios Terry Papoutsakis (2024). Flux balance analysis and peptide mapping elucidate the impact of bioreactor pH on Chinese Hamster Ovary (CHO) cell metabolism and N-linked glycosylation in the Fab and Fc regions of the produced IgG. Metabolic Engineering (Accepted). 

3. Indrani Nandi, Rohini Kumari, Kajal Kachhawaha, Sumit K. Singh, Pranjal Chandra (2024) Electrochemical Sensor based on MXene Nanosheet-Gold NanoUrchin Hybrid as a Superoxide dismutase mimic for real-time detection of superoixde anions released from living cells. ACS Applied Nano Materials (Accepted)

4. Santanu Singh, Kajal Kachhawaha, Sumit K. Singh* (2024) Comprehesive approaches to preclinical evaluation of monoclonal antibodies and their next-generation derivatives. Biochemical Pharmacology (Accepted)
5. Santanu Singh, Rohan Shah, Sumit K. Singh* (2024) Comparative analysis of shotgun and targeted proteomics: Insights for biomarker research.Protein Biomarkers: Discovery and Application in clinical diagnostics (Accepted)
6. Kajal Kachhawaha, Rohan Shah, Sumit K. Singh* (2024) Clinical sample considerations in proteomics-based biomarker studies: advancing precision medicine. Protein Biomarkers: Discovery and Application in clinical diagnostics (Accepted)
7. Khyati Joshi, Rohan Shah,Sumit K. Singh* (2024). Experimental design in proteomic mass spectrometry studies for biomarker discovery. Protein Biomarkers: Discovery and application in clinical diagnostics (Accepted)
8. Sahil Khan, Aryaman Joshi, Sumit K. Singh*, Modeling of N-linked glycosylation: Advances and Challenges in predicting glycan structures during biomanufacturing. Preprint: Doi: 1022541/au.168382757.72018049/v1
9. Niharika G. Jha, Daphika S. Dkhar, Sumit K.Singh, Shweta J. Malode,Nagaraj P. Shetty, Pranjal Chandra (2023) Engineered biosensors for diagnosing multi-drug resistance in microbial and malignant cells. Biosensors (Accepted)
10. Aryaman Joshi, Timir Tripathi, Sumit K.Singh, Aditya K.Padhi(2022).Computational approaches to develop engineered therapeutics against SARS-CoV2. ACS Biochemistry (Accepted).
11. Kajal Kachhawaha, Santanu Singh, Khyati Joshi, Priyanka Nain, Sumit K. Singh* (2022). Bioprocessing of recombinant proteins from Escherichia coli Inclusion bodies: Insights from structure-function relationship for novel applications. Journal of Preparative Biochemistry and Biotechnology (Accepted) IF: 3.12 
12. Sumit K. Singh, Deepak Kumar, Saurabh Nagpal, Sunil K. Dubey, Anurag S. Rathore (2022).  A charge variant of bevacizumab offers enhanced FcRn-dependent pharmacokinetic half-life and efficacy. Pharmaceutical Research (In press). IF: 4.91
13. Sumit K Singh, Kelvin H Lee (2021) Characterization of monoclonal antibody glycan heterogeneity using hydrophilic interaction liquid chromatography-mass spectrometry. Frontiers in Bioengineering and Biotechnology (In press) DOI : 10.3389/fbioe.2021.805788 IF: 6.06
14. Rozaleen Dash, Sumit K Singh, Narendra Chirmule, Anurag S Rathore (2021). Assessment of functional characterization and comparability of biotherapeutics: A review. The AAPS Journal (In press) DOI: 10.1208/s12248-021-00671-0. IF: 4.46 

Publications before joining IIT (BHU)

1. Sumit K Singh, Deepak Kumar, Anurag S Rathore (2020). Understanding Oxidation Propensity in GCSF and Assessment of its Safety and Efficacy. Pharmaceutical Research, 37, (207). IF: 4.91
2. Sumit K Singh, A Mishra, N Chirmule, G Goel, Anurag S Rathore (2020) Modulation of granulocyte colony stimulating factor conformation and receptor binding by methionine oxidation. Proteins: Structure, Function and Bioinformatics, 1-13. IF: 4.08
3. Sumit K Singh, D Kumar, Anurag S Rathore (2021) LC-MS based case-by-case analysis of the impact of acidic and basic charge variants of bevacizumab on stability and biological activity. Scientific Reports, 11(1), 1-15. IF: 4.99
4. Avinash Mishra, Rucha Patil, Sumit K Singh, Priyanka Dalal, Anurag S. Rathore (2020) Mechanistic explanation of structural and functional changes induced by methionine mutation in G-CSF protein. Current Research in Biotechnology, 2, 37-44. IF: 5.19
5. Sumit K Singh, Avinash Mishra, Divyanshi Yadav, Niharika Budholiya, Anurag S Rathore (2019) Understanding the mechanism of copurification of “difficult to remove” host cell proteins in rituximab biosimilar products. Biotechnology Progress, e2936. IF: 2.90
6. Sumit K Singh, G Goel, Anurag S Rathore. (2019). A novel approach for protein identification from complex cell proteome using modified peptide mass fingerprinting algorithm. Electrophoresis,40, (23-24), 3062-3073. IF: 3.59
7. Anurag S Rathore, Dinesh K Yadav, Shyam S Pandey, Sumit K Singh, Deepak Kumar (2018). Preclinical Evaluation of Product Related Impurities and Variants.  Biopharm International, 31 (2), 26-31.
8. Sumit K Singh, Deepak Kumar, Anurag S Rathore. (2017). Determination of critical quality attributes for a biotherapeutic in the QbD paradigm: GCSF as a case study. The AAPS Journal, 19(6), 1826-1841. IF: 4.46
9. Neh Nupur, Sumit K Singh, Gunjan Narula, Anurag S Rathore (2017). Assessing analytical comparability of biosimilars: GCSF as a case study. Journal of Chromatography B, 1032, 165-171. IF :3.38
10. Sumit K Singh, Vishwanath Hebbi, Anurag S Rathore, Ira S Krull (2016). Practical Considerations of Capillary Electrophoresis–Mass Spectrometry for Analysis of Biotherapeutics. LC-GC North America, 34(9), 720-728.
11. Gunjan Narula, Vishwanath Hebbi, Sumit K Singh, Anurag S Rathore. (2016). Glycosylation in mAb therapeutic products: analytical characterization and impact of process. LC-GC Europe, 29(7), 385-392.
12. Sumit K Singh, G Narula, AS Rathore (2016). Should charge variants of monoclonal antibody therapeutics be considered critical quality attributes? Electrophoresis, 37(17-18), 2338-2346.
13. Sumit K Singh, Anurag S Rathore (2015). Use of multivariate data analysis in bioprocessing.  Biopharm International, 28(6), 26-31.
14. Anurag S. Rathore, Sumit K Singh, Mili Pathak, Erik K. Read, Kurt A. Brorson, Cyrus D. Agarabi, Mansoor Khan (2015) Fermentanomics: Relating quality attributes of a monoclonal antibody to cell culture process variables and raw materials using multivariate data analysis, Biotechnology Progress, 31 (6), 1586-1599.
15. Debamitra Chakravorty, Sumit K. Singh, Parameswaran Saravanan, Sanjukta Patra (2013). Design of lead peptide drugs from mushroom targeting cysteine proteases. Medicinal Chemistry Research 22(4), 2038-2049.
16. Sumit K Singh, Anurag S Rathore (2014). Clinical and Non-Clinical aspects of biosimilar development .BioQuality 19(9-10),2-6 
17.  ​Douglas Nmagu, Sumit K. Singh, Kelvin H. Lee. Creation of monoclonal antibody expressing CHO cell lines grown with sodium butyrate and characterization of resulting antibody glycosylation. Methods in Enzymology (2021).

​

18. Anurag S Rathore, Sumit K Singh, J Kumar, G Kapoor. (2018) Implementation of QbD for Manufacturing of Biologics—Has It Met the Expectations? Biopharmaceutical Processing, 1051-1073.

​

19. Anurag S Rathore, Sumit K Singh (2017). Chemometrics Applications in Process Chromatography.  Preparative Chromatography for Separation of Proteins, 479- 500.
20. Anurag S Rathore, Sumit K Singh (2016). Production of protein therapeutics in the quality by design (QbD) paradigm. Protein Therapeutics, 41-67.

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21. Anurag S Rathore, Sumit K Singh, Neh Nupur, Gunjan Narula. (2016). Role of proteomics in characterization of biosimilar products. Biomarker Discovery in the Developing World: Dissecting the Pipeline for Meeting the Challenges, 83-97.

List of Important Presentations/meetings

After Joining IIT-BHU

1. Sumit K. Singh. Identification of critical quality attributes of biotherapeutics: Case studies. International Conference on Advancements in Diagnostics Technologies, Global Healthcare Monitoring. MNNIT Allahabad, Prayagraj (November 2024)
2. Sumit K. Singh. Development of all-natural Silk protein-anthocyanin-based restorative hair-dye formulation. CGMFP, Raipur (July 2024).
3. Jayanth Venkatarama Reddy, Thomas Leibiger, Sumit K. Singh, Kelvin Lee, Eleftherios Papoutsakis, and Marianthi Ierapetritou.  Effect of critical process parameters on site specific N-linked glycosylation of VRC01 produced in CHO. 28th Meeting of European Society for Animal Cell Technology ( June 2024), Edinburgh.
4. Sumit K. Singh. Clinical Assay development: Challenges for translation. Advances in Proteomic Technologies Conference, IIT Mumbai (February 2024) [coordinator/moderator of panel discussion]
5. Sumit K. Singh. Mapping mAb critical quality attribute: Modeling glycosylation from mass spectrometry data. Advances in Proteomic Technologies conference, IIT Mumbai (February 2024). [ECR Talk]
6. Sumit K. Singh, Characterization of proteins and polymers using mass spectrometry and other analytical methods, Department of polymer and process engineering,  IIT Roorkee (December 2023)- [INVITED TALK]
7. Sumit K.Singh, PTM  characterization of biotherapeutics using native mass spectrometry, CoE CBT Course Series, 2023, IIT Delhi  (December 2023) [INVITED TALK]
8. Sumit K. Singh, Introduction to native mass spectrometry, CoE CBT Course series, 2023, IIT Delhi (December 2023) [Keynote Lecture]
9. JV Reddy, Sumit K Singh, T Leibiger, E Papoutsakis, M Ierapetritou. Insights into the effect of bioreactor pH on Chinese Hamster Ovary cell metabolism and site-specific N-linked glycosylation of VRC01 mAb, AIChE Annual Meeting, Orlando, FL (November 2023)
10. Sumit K. Singh, Exploring high-resolution mass spectrometry for biopharma applications, SCIEX Webinar (online) [Invited talk] (September 2023)
11. Sumit K. Singh, Modeling N-Glycosylation in Chinese Hamster Ovary (CHO) cells expressing VRC-01 antibody. 9th Metabolic Pathway Analysis Conference, Seoul, South Korea (July 2023) [Lightning Talk]
12. Sumit K.Singh, MolDev techtalk, Molecular devices, Bangalore
13. Sumit K.Singh, Biopharma Workshop, CoE SCIEX India, Bangalore, 2023
14. Sumit K. Singh, Technqiues to study proteins to proteome, Hands-on-training on biological and molecular techniques, Department of Zoology, MMV, Banaras Hindu University, March 2023 [Invited talk]
15. Sumit K. Singh, Activity loss due to methionine oxidation in GCSF: mechanism and control, 8th International Bioprocessing India conference,  December 2022, CSIR-NCL Pune [Invited Talk]
16. Sumit K. Singh, Characterization of primary structure and post-translational modifications using multi-attribute monitoring workflows, Center of Biopharmaceutical Technology Course Series, December 2022,  IIT Delhi, India [Invited Talk]
17. Sumit K. Singh, Characterization of biopharmaceuticals using peptide mapping workflows, Center of Biopharmaceutical Technology Course Series, December 2022, IIT Delhi, India [Invited Talk]
18. Sumit K. Singh. Theory and practice of native mass spectrometry, Center of Excellence for Biopharmaceutical Technology Course Series, December 2022, IIT Delhi, India [Invited Talk]
19. Sumit K. Singh, Bioprocessing of mAbs:  producing safe and efficacious biologics. Expanding Horizons of Downstream Processing, July 2022, Kalasaligam Academy of Research and Education, Krishnankoil, Tamil Nadu, India [INVITED TALK]
20. Sumit K. Singh, Concepts in liquid chromatography: Instrumental aspects, Hands-on Training Programme on Bioprocessing, June 2022, IIT Delhi, India  [INVITED TALK]
21. Sumit K. Singh, Controlling Critical Quality Attributes of Biopharmaceutical products, Center of Excellence for Biopharmaceutical Technology Course Series, April 2022, IIT Delhi, India  [INVITED TALK]
22. Sumit K Singh, Case studies of mass spectrometry-based characterization of biopharmaceutical products, Center of Excellence for Biopharmaceutical Technology Course Series, December, 2021, IIT Delhi, India  [INVITED TALK]
23. Sumit K Singh, Characterization of primary structure and post-translational modifications of biopharmaceuticals, Center of Excellence for Biopharmaceutical Technology Course Series, December, 2021, IIT Delhi, India  [INVITED TALK]
24. Sumit K Singh, Multivariate Data Analysis for Biotech Processes:  Concepts and Case Studies, FDP on Recent Developments in the field of Computational Biology, Institute of Engineering and Management, November 2021, Kolkata, India [INVITED TALK]

Before joining IIT-BHU

1. Sumit K Singh, Kelvin H Lee. Monoclonal antibody glycan analysis using mass spectrometry.  American Chemical Society National Meeting, August 2020, Virtual.
2. Sumit K Singh, D Kumar, AS Rathore. Activity loss due to methionine oxidation in GCSF: Mechanism and control, Gordon Research Conference on Biotherapeutics and Vaccine Development, January 2019, Galveston, Texas[INVITED TALK]. 
3. Sumit K Singh, D Kumar, DK Yadav, AS Rathore. Identification of critical quality attributes of biotherapeutics: GCSF as a case study.  American Chemical Society National Meeting, April 2017, San Francisco
4. Sumit K Singh, AS Rathore. Fermentanomics: Relating quality attributes of a monoclonal antibody to cell culture process variables and raw materials using multivariate data analysis. American Chemical Society National Meeting, April 2017, San Francisco. 
5. Sumit K. Singh, Anurag S. Rathore, Multivariate data analysis for biotech processes: Industrial case studies, American Chemical Society National Meeting, April 2017, San Francisco
6. Sumit K Singh, AS Rathore. Modulation of granulocyte colony stimulating factor conformation and receptor binding by methionine oxidation, Bioprocessing India Conference 2018, IIT Delhi
7. Sumit K Singh, D Kumar, AS Rathore, Investigating the effect of oxidation of granulocyte colony stimulating factor on biological activity using experimental and computational approach, American Chemical Society National Meeting, April 2017, San Francisco
8. Sumit K Singh, AS Rathore. Host Cell Protein Analysis by HPLC/CE-ESI-TOF-MS. Targeted Proteomics workshop and International Symposium, December 2015, IIT Mumbai [INVITED TALK]. 

List of Important Posters

1. Sumit K Singh, AS Rathore. Modulation of granulocyte colony stimulating factor conformation and receptor binding by methionine oxidation. Bioprocessing India Conference, December 2018, IIT Delhi.
2. Sumit K Singh, D Kumar, AS Rathore. Investigating the effect of oxidation of granulocyte colony stimulating factor on biological activity using experimental and computational approach. American Chemical Society National Meeting, April 2017, San Francisco.
3. Sumit K Singh, D Chakravorty, S Patra. In silico prediction of mechanism of action of cysteine protease inhibitors from mushroom. Annual conference of Association of Microbiologist of India, December 2010, BIT Mesra.