m.debnath.bce's picture
Dr. Mira Debnath Das
Professor
School of Biochemical Engineering IIT BHU
m.debnath.bce@iitbhu.ac.in
9450387552
Area of Interest: 
Enzyme Engineering, Genetic Engineering, Microbial Engineering, Fermentation Technology, Downstream Processing, Instrumentation and Process Control

B. Tech. Jadavpur University, Kolkata, India 1978 Food Tech & Biochemical Engineering I Class 1st     
University Gold Medal,
Lewis Pasteur Gold Medal,
Hira Lal Roy Centred Gold Medal 
M.Tech. Jadavpur University, Kolkata, India 1980 Food Tech & Biochemical Engineering I Class 2nd
Ph.D. Jadavpur University, Kolkata, India 1987 Enzyme Engineering -
National Award Department of Biotechnology  Board, Govt. of India 1988-89 Genetic Engineering National Associate
1981-82 Senior Research Fellow Food Technology and Biochemical Engineering; Jadavpur University. Project work was on “Alcohol production from Low-Cost starchy raw materials.” Supervisor : Prof. S.K. Majumdar and Prof. B.N. Srimani.
1982-85 Senior Research Fellow of council of Scientific and Industrial Research (C.S.I.R) Food Technology and Biochemical Engineering; Department; Jadavpur University; Ph.D. thesis on “Production of Glucose Isomerase from Streptomyces Kanmyceticus. Supervisor : Prof. S.K. Majumdar
1985-90 Lecturer School of Biochemical Engineering, IIT (BHU), Varanasi
1990-1998 Senior Lecturer School of Biochemical Engineering, IIT (BHU), Varanasi
1998-2016 Professor School of Biochemical Engineering, IIT (BHU), Varanasi
2009-2013 Coordinator School of Biochemical Engineering, IIT (BHU), Varanasi
2016- Onwards Sr. Professor (HAG) School of Biochemical Engineering, IIT (BHU), Varanasi
  • DPGC Convener from 2014 to 2018 in School of Biochemical Engineering IIT(BHU), Varanasi. DUGC convener 2018-continuing.
  • Coodinator from 2009 to 2013 in School of Biochemical Engineering IT(BHU)& IIT(BHU), Varanasi.
  • DBT task force Member from 2013 - 2018
  • Member of Kendriya Vidyalaya   B.H.U School advisory committee.
  • Member of admission committee in B.H.U central Girls” School.
  • Warden IT BHU Girls hostel from 2003 to 2006.
  • DRDO Recruitment Cell expert member.
  • Recipient of University Gold Medal, Jadavpur University, Kolkata, India, 1978.
  • Recipient of Hira Lal Roy Centred Gold Medal, Jadavpur University, Kolkata, India, 1978
  • Recipient of Leuis Pasteur Gold Medal, Jadavpur University, Kolkata, India, 1978
  • Recipient of D.B.T. National Associateship Award, 1988-89
  • Recipient   of CSIR (SRF) 1982-85
  • DBT task force Member from 2013 - 2017
  • U.G.C Major Project (Improvement strategy for operational stability of penicillin Acylase enzyme for production of semi-synthetic β-lactum antibiotics) on Penicillin Acylase, 1-1-2005 to 31-12-2007.
  • AICTE Major Project (Rapid detection and enumeration approach of pathogenic bacteria using solid phase in food and water industries) 30-12-2006 to 30-12-2009
  • Co-Pi of sprouting proposal. IIT(BHU)
Name of Research Scholar Research Topics Status
Dr. M.S. Raju Studies on Microbial degradation of hydrocarbons. Completed
Dr. Abha Mishra High fructose syrup production using multi-enzyme system Completed
Dr. Sujoy Banerjee  Studies on penicillin acylase production. Completed
Dr. Ravi G. Kumar Production, Purification and Characterization of Ribonuclease from Aspergillus niger (Folding and Biological Aspects) Completed
Dr. Neeraj Gupta
 
Microbial D-amino acid oxidase: Production, Purification, Characterization & its   biological application. Completed
Mr. Shiv Kumar Verma Production of Bioactive Compound from Endophytic fungus. Ongoing
Mr. Anand Kumar Immunological studies of lipoprotein of Salmonella typhi as a vaccine candidate. Completed
Mr. Ashish Production, Optimization and Characterization of Bio-surfacatant from an adaptive strain C. tropicalis MTCC 230 and its application. Completed
Mr. Debashish Dutta  Bioprocess strategy development for enhanced anti-fungal protein production Submitted
Mr. Moti Lal
 
Study and application of bioactive metabolites from endophytic bacteria. Ongoing
Mr. Rahul (Ongoing)            
 
Encapsulation of hydrophobic drugs
 
Ongoing
  • Cloning of pac gene in E.Coli. and optimization of some process parameter in batch mode.
  • SCP production from petroleum waste hydrocarbons.
  • Studies on glucose isomerase production from Bacillus species.
  • Studies on penicillin G acylase activities in E.Coli.
  • Studies on glucose isomerase production from Streptomyces griseus and fructose separation from equimolor mixture of glucose and fructose.
  • Studies on the use of indigenous membranes in a membrane bioreactor.
  • Enzymatic detoxification of parathion (insecticide).
  • Production of glucoamylase by Aspergillus oryzae MTCC 152 in solid state fermentation.
  • Studies on effect of solvent on lipase production from Rhizopus arrhizus.
  • Studies on Biofertilizer production and phosphate solubilization by A.niger.
  • Biocatalytic activity of lipases in presence of water and organic solvent.
  • Candida cylindricea lipase-mediated synthesis of isoamyl butyrate.
  • Studies on production, purification and effect of organic solvent on penicillin acylase of E.coli.
  • Studies on Microbial Detoxification of Parathion.
  • Development of Rapid method for Detection of E. coli in Drinking water.
  • Biodiesel Production by Lipase enzyme from different vegetable oil sources.
  • Enhanced production of Bio-surfactant by Candida tropicalis MTCC 230.
  • Optimization of process parameters for a packed bed reactor for the production of 6-amino penicillinic acid.
  • Optimization of factors affecting the efficiency of packed bed column for sucrose conversion by immobilized invertase.
  • Production of amylase with enhanced thermo-stabilty.
  • Studies on antifungal peptides.
  • Microbial up-streaming and down-streaming of insulin.
  • Study on penicillin amidase.
  • Penicillin G amidase immobilization and its kinetic study.
  • Production of seudrophores.
  • Molecular and immunological characterization of lp1, the 34 kda outer membrane lipoprotein of Pseudomonas aeruginosa.
  • Lipase immobilization and its kinetic study.
  • Lactic acid production.
  • Fabrication and comparative study of silk fibroin.
  • Comparative studies of formation of chitosan nanoparticles using bio-surfactant.
  • Green synthesis of silver nanoparticles from Tectona grandis seeds’ extract.
  • Adsorption of Ampicillin on functionalized multi-walled carbon nanotubes.
  • Member of editorial board: African Journal of Biochemistry and Research.
  • Member of editorial board: International Journal of Biology and Chemical Science.

International:

  • Isomerase production by Streptomyces griseus NCIM2020; A Mishra and M. Debnath (Das Glucose isomerase activity of Streptomyces Kanmyceticus. Mira Debnath and S.K. Majumdar, Biotechnology Letters, Vol. 7, No. 5, 1985. (IF-1.6)
  • Effect of minerals on glucose isomerase production by Streptomyces Kanmyceticus. Mira Debnath (Das) and Saroj Kanti Majumdar. Applied Microbiology and Biotechnology, 26, 1987. (IF-3.6)
  • Effect of some important factors on production of glucose isomerase, M. Debnath (Das) and S.K. Majumdar; Journal of Microbiology and Biotechnology; Vol. 6, part 2. 1991. (IF-1.3)
  • Molecular cloning of penicillin acylase gene and process optimization of penicillin acylase enzyme using recombinant cell. S. Bhattacharya; P.K. Ranjekar and M. Debnath (Das). Congress on Cell and Tissue Culture; San Diego; CA-June 5-9, 1993; U.S.A.
  • Production, process optimization and immobilization of penicillin acylase enzyme from E.Coli ATCC 11105; S. Bhattacharya, M.Debnath (Das), P.K. Ranjekar. Enzyme Microbial Technology, vol. 15, 1993. (IF-3.0)
  • Effect of pH on simultaneous saccharification and isomerization by glucoamylase and glucose isomerase; Abha Mishra and Mira Debnath (Das); Journal of Applied Biochemistry and Biotechnology, Vol. 102-103,  2002. (IF-1.9)
  • Role of organic solvents in the improvement of operational stability of penicillin G acylase, an industrially important biocatalyst, Sujoy Banerjee, Sanjay Gupta, Mira Debnath, Brazilian Archives of Biology and Technology.
  • Continuous Production of 6-amino penicillanic acid (6-APA) by agarose immobilized penicillin acylase in a packed column reactor, Sujoy Banerjee and Mira Debnath, Chemical and Biochemical Eng. Q. Vol.21(3) 265-269, (2007)
  • Organic solvents to enhance the activity and stability of penicillin acylase as a robust biocatalyst, Sujoy Banerjee and Mira Debnath Cameroon journal of experimental biology. 2: 83-87.
  • Extracellular ribonuclease from Aspergillus niger: process optimization for production, Gundampati R.K, Debnath M, International Journal of Engineering and Technology Vol. 1, No. 4 , October, 2009
  • Extracellular ribonuclease production from Aspergillus niger: process optimization by biostatistical analysis, Gundampati R.K, Debnath M, World Academy of Science, Engineering and Technology, Volume 56 August 2009 ISSN 2070-3724
  • Surface charge based rapid method for detection of microbial contamination in drinking water and food products. Kandpal.M, Gundampati. R. K., Debnath M. World Academy of Science, Engineering and Technology, Volume 58 October 2009 ISSN 2070-3724.
  • HU Equilibrium unfolding of A. niger RNase: pH dependence of chemical and thermal denaturation. Gundampati R.K Anurag Sharma, Moni Kumari, M V Jagannadham, Debnath M*  European Biophysics Journal. 40 (2011) 923-935.
  • Extracellular ribonuclease production from Aspergillus niger: process optimization by biostatistical analysis. Gundampati R.K, Swati Singh, Neeraj Gupta, Debnath M*, International Journal of Biotechnology and Biochemistry 7 (2011) 279-291.
  • Protein-Protein Docking on Molecular Models of Aspergillus niger RNase and human Actin: Novel target for Anticancer Therapeutics. Gundampati R.K, Chikati Rajasekhar, Moni Kumar, Anurag Sharma, D. Devi Pratyush, M V Jagannadham, Chitta Suresh Kumar, Debnath M*. Journal of Molecular Modeling 18 (2012)
  • Enhancement of D-amino acid oxidase by Trigonopsis variabilis. Neeraj Gupta, Gundampati R.K, Debnath M, APCBEES Procedia , 2 (2012) 115-119.:653-62.
  • Molecular modeling, docking and dynamics simulations of A.niger RNase from   Aspergillus niger ATCC26550 for potential prevention of Human Cancer. Gundampati Ravi Kumar*, Rajasekhar Chikati, Santhi Latha Pandrangi, Kirti Sonkar, Chitta Suresh Kumar,  Sunita Saxena, M V Jagannadham, Mira Debnath (Das) *. Journal of Molecular Modeling. DOI 10.1007/s00894-012-1587-9, (2012).
  • Insilico studies onn complete inhibition of Trypanothione Reductase of Leishmania infantum by γ-sitosterol and Antcin-α: Novel target for anti-leishmanial activity. Ravi Kumar Gundampati, Shraddha Sahu, Kirti Shila Sonkar, Mira Debnath and Medicherla V. Jagannadham. American Journal of Biochemistry and Molecular Biology. DOI: 10.3923/AJBMB, (2013).
  • Modeling and Molecular docking studies on RNase Aspergillus niger and Leishmania donovani actin: anti-leishmanial activity. Ravi Kumar Gundampati, Shraddha Sahu, Kirti Shila Sonkar, Avinash Kumar Srivastava, Mira Debnath and Medicherla V. Jagannadham. American Journal of Biochemistry and Biotechnology. DOI: 10.3844/ajbbsp (2013) 318-328.
  • Antimicrobial activity of Endophytic fungal Isolate in Argemon mexicana; A traditional Indian Medicinal Plant. Shiv Kumar Verma, Anand Kumar, Ashish, Mira Debnath (Das). IJIRSET. 3 (2014) 10151-10162.
  • Multifactorial Approach to Biosurfactant production by Adaptive Strain Candida tropicalis MTCC 230 in the presence of Hydrocarbons. Ashis Verma, Niroj gupta, Shiv Kumar Verma. Mira Debnath (Das). J. Surfact. Deterg., 18(1), 2015 145–153.
  • Biodegradation of Synthetic Dye by Endophytic Fungal Isolate in Calotropis procera Root. Shiv Kumar Verma, Anand Kumar, Moti Lal, Mira Debnath. Int J Appl Sci Biotechnol, Vol 3(3) (2015) 373-380.
  • Thermo Stability Study of Crude Amylase from Bacillus Isolate. Moti Lal, Shiv Kumar Verma, Vipin Mishra, Mira Debnath. Int J Appl Sci Biotechnol, Vol 3(3) (2015) 427-430.
  • Study of bioactive metabolites from endophytic bacteria isolate. Moti lal, Neelam, Shiv kumar, Mira debnath (Das). International Journal of Scientific and Engineering Research, 7(6) (2016) 598-606.
  • Molecular docking and Simulation studies to give insight of surfactin amyloid interaction for destabilizing Alzheimer's Aβ42 Protofibrils. Ashish Verma, Akhil Kumar, Mira Debnath (Das). Medicinal Chemistry Research, August 2016, Volume 25, Issue 8, pp 1616–1622
  • Molecular and immunological characterization of lp1, the 34 kda outer membrane lipoprotein of Pseudomonas aeruginosa. Ananad Kumar, Sada shiva, Mira Debnath (Das). Biologia. Volume 71, Issue 7, Pages 743–747
  • Optimization and Partial Characterization of Intracellular Anticandidal Protein from Aspergillus giganteus MTCC 8408 Using Taguchi DOE. Debashis Dutta, Mira Debnath Das Journal Bioengineered Pages 1-13
  • Preparation and Biochemical Property of Penicillin G Amidase-Loaded Alginate and Alginate/Chitosan Hydrogel Beads.  Neh Nupur, Ashish, Mira Debnath (Das) Recent Patents on Biotechnology. 2016. Vol.10(1);121-132
  • Antimicrobial activity of extract from endophytic fungus in Calotropis procera root. Shiv Kumar Verma, Anand Kumar, Moti Lal and Mira Debnath (Das). Res.  Environ. Life Sci. 9(2) 212-216 (2016).
  • Optimization of process parameters for Production of antimicrobial metabolites by an Endophytic fungus     Aspergillus sp. CPR5 Isolated from Calotropis procera root. Shiv Kumar Verma, Moti Lal, Mira Debnath (Das) Asian J. Pharm. Clin. Res. (Accepted, IP- 0.48)
  • Expression, Purification and Evaluation of recombinant lipoprotein of Salmonella typhi as a vaccine candidate. Anand Kumar, Subir Kundu, Mira Debnath (Das) Biologicals (Accepted)
  • Expression and optimization of recombinant lipoprotein of Salmonella typhi in prokaryotic host using RSM. Anand Kumar, Subir Kundu, Mira Debnath (Das), Research journal of Biotechnology (Accepted)
  • Studies of Bioactive Metabolites from Endophytic Bacterial Isolate. Moti Lal, Neelam, Shiv Kr. Verma, Mira Debnath (Das). International Journal of Scientific & Engineering Research, Vol 7, Issue 6, June-2016 598-606. (IP- 3.8, Thomson Reuters).
  • Bioactive metabolites obtained from endophytes bacterial isolates. Moti Lal, Neelam Tia, Debashis Dutta, Mira Debnath (Das). International Journal of Innovative Research in Science, Engineering and Technology. Vol. 5, Issue 7, July 2016. 14051- 14063. (IP- 6.3)
  •  Thermostability study of crude amylase from bacillus isolates. Moti Lal, Shiv Kumar Verma, Vipin Mishra and Mira Debnath (Das). Int. J. Appl. Sci.  Biotechnol, 2015 Vol 3(3): 427-430. (IP- 1.43).
  • Homology modeling of outer membrane lipoprotein of Salmonella typhi.  Anand Kumar, Shiv Kumar, SubirKundu, Mira Debnath (Das). Res.  Environ. Life Sci, 2016 9(7) 900-902
  • Characterisation of   Bioactive Metabolites from Endophytic Bacterial Isolate. Lal Moti,  Neelam Tia, Shiv Kr. Verma, Dutta Debashis and Mira Debnath (Das). Research Journal of Biotechnology.  Vol.  12 (1), January 2017. (IP- 0.2, SCI)
  • Debashish dutta, Mira Debnath (Das), Optimization of Mycelial Biomass and Antifungal Protein from Aspergillus giganteus Using Taguchi DOE in Submerged Fermentation. Biocontrol (2017) Vol.22, No.2 (IF:0.7)
  • Optimization and Partial Characterization of Intracellular Anticandidal Protein from Aspergillus giganteus MTCC 8408 Using Taguchi DOE. Debashis Dutta, Mira Debnath Das Journal Bioengineered 2016, Pages 1-13
  • Study of bioactive metabolites from endophytic bacteria isolate. Moti lal, Neelam, Shiv kumar, Mira debnath (Das). International Journal of Scientific and Engineering Research, 7(6) (2016) 598-606. ((I.F 3.8))
  • Optimization of process parameters for Production of antimicrobial metabolites by an Endophytic fungus Aspergillus sp. CPR5 Isolated from Calotropis procera root. Shiv Kumar Verma, Moti Lal, Mira Debnath (Das) Asian J. Pharm. Clin. Res. (Accepted, IF- 0.48) (SCOPUS)
  • Studies of Bioactive Metabolites from Endophytic Bacterial Isolate. Moti Lal, Neelam, Shiv Kr. Verma, Mira Debnath (Das). International Journal of Scientific & Engineering Research, Vol 7, Issue 6, June-2016 598-606. (Thomson Reuters).(I.F 3.8)
  • Bioactive metabolites obtained from endophytes bacterial isolates. Moti Lal, Neelam Tia, Debashis Dutta, Mira Debnath (Das). International Journal of Innovative Research in Science, Engineering and Technology. Vol. 5, Issue 7, July 2016. 14051- 14063. (IF- 6.3)
  • Effect of carbon and nitrogen sourceson Mycellial Biomass and biosynthesis of Antifungal protein from Aspergilus giganteusin submerge fermentation, Debashis Dutta & Mira Debnath (2017), JSIR Vol.76(06) 369- 375
  • Biosynthesis of Low Molecular weight of Antifungal protein from A giganteus in Batch fermentation and in vitro assay. Debashis Dutta & Mira Debnath Das (2018), Journal of Biocontrol Science, Vol 23, No.2,2018.
  • Effect of C/N ratio and microelements on nutrient dynamics and cell morphology in submerged fermentation of A.giganteus using Taguchi DOE , Debashis Dutta & Mira Debnath(2017) 3 Biotech May 2017, 7:34
  • Pre clinical comperative study of microbial derived surfactant with surventa for treatment of respiratory distress syndrome. Ashish, Jitendra Sing Parihar, Niraj Sharma and Mira Debnath(2017), Current Trends in Biotechnology and Pharmacy 11(3), 268- 277
  • Expression, purification,and evaluation of recombinant lipoprotein of Salmonella typhi as a vaccine candidate. Anand Kumar, Subir Kundu and Mira Debnath  (2017), Biologicals, 46, 108-113.
  • Effects of the probiotics Lactococcus lactis ( MTCC-440) on Salmonella enteric serover Typhi in coculture study. Anand Kumar, Subir Kundu , Mira Debnath (2018), Microbial Pathogenesis 120, 42-46.
  • Homology modeling of outer membrane lipoprotein of salmonella typhi. Research in     Environmental and Life Sciences. Anand Kumar, Shiv Kumar, Subir Kundu, Mira Debnath(Das)(2016)
National
  • Improvement strategy for operational stability of penicillin acylase for 6-APA production, Sujoy Banerjee and Mira Debnath, Indian Journal of Microbiology Vol 46, No. 2 June 2006, pp 175-180. (IF-0.5)
  • Esterification reactions catalysed by surfactant coated Rhizopus arrhizus lipases, P.Sasi, Rohit R Mehrortra and M.Debnath, Indian Journal of Biotechnology. Vol.5. 2006. (IF-0.5)
  • Optimization of media composition for D-amino acid oxidase production by Trigonopsis variabilis using biostatistical analysis. Neeraj Gupta, Ravi Kumar Gundampati and M Debnath. Indian Journal of Biochemistry & Biophysics. 49 (2012) 272-278.
  • Structural Elucidation of Bioactive Secondary Metabolites from Endophytic Fungus. Shiv Kumar Verma, Moti Lal, Mira Debnath (Das) Indian Journal of pharmaceutical Science. (IP- 1.7, SCI).
Conferences
  • Enhancement of D-amino acid oxidase by Trigonopsis variabilis Neeraj Gupta, Ravi Kumar Gundampati, Debnath M, accepted in International Conference on Biotechnology & Food Technology (ICBFT-2012), Bangkok, Thailand & will be published in the APCBEFS Procedia Journal under Elsevier.
  • Antimicrobial activity of endophytic fungi residing in Argemone maxicana root Shiv K. Verma, Ashish, Anand K., Ravi K. Sharma, Avinsh Srivastava, Moti Lal, Mira Debnath (Das)*1(2013), pp.65-67, Biosangam MNNIT, Allahabad.
  • Antibacterial property of Endophytic fungi in Argemone maxicana stem. Shiv K. Verma, Ashish, Anand K, Mira Debnath. (Das)*; Conference title-New approaches in food sequrity and value addition:Technological and genetic options, organized by post graduate department of biotechnology, RBS Engineering technical Campus Bichupuri Agra. Page No. 82.
  • Oral presentation on Comparative study of Bacillus subtillis sp. and Candida tropicalis MTCC 230 for production of surfactin (2014) Ashish. Mira Debnath (Das), national conference genesis HBTI, Kanpur, HCO 03 page 30.
  • Modelling and Optimization of Batch Culture for the Mycelial Growth and Antifungal Protein Production By Aspergillus Giganteus. International Conference on Biomolecular Engineering AIChE, Singapore, 2016.
  • Production and Characterization of Biosurfactant from an adaptive strain C.tropicalis MTCC 230, International Conference on Beneficial Microbes, Thailand, 2016.
Book/Book Chapter
  • Debashis Dutta, Mira Debnath Das*, Book: Production and Optimization of Anti-fungal Protein Using Taguchi DOE, LAP Lambert Academic Publishing (GmbH, Germany), 2016. ISBN: 3659936952.
  • Debashis Dutta, Mira Debnath Das*, Book Chapter: Food Irradiation Technology: A Novel Aspect for the Future in Food Process Engineering and Quality Assurance, CRC Press (Taylor and Francis), 2016. ISBN: 9781771885768.
  • Ashish, Mira Debnath Das, Book: Bio-surfactant Production and Application from an adaptive strain. ISBN-10: 365992721X, LAP Lambert Academic Publishing (September 15, 2016).
Sequences submitted
  • Aspergillus niger 18S rDNA sequence, (DNA Data bank of Japan) ACCESSION No. LC062385
  • Trichoderma  asperellum 18S r DNA Sequence, (DNA Databank of JAPAN) ACCESSION No. LC063169
  • Salmonella enterica subsp. enterica serovar Typhimurium str.SL1344 st2 gene for surface Lipoprotein, complete cds in DDBJ (DNA Data Bank of Japan) ACCESSION No. LC069355